Regulatory

Part:BBa_K3661006:Design

Designed by: Weixuan Lu   Group: iGEM20_CPU_CHINA   (2020-10-23)


JTi2(NF-kb induced promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we have considered about changing the repeats number in the binding sites, and eventually decided to apply 5 times as the old part did.


Source

Homo genome,sequence found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/NM_001078 Iademarco MF, McQuillan JJ, Rosen GD, Dean DC. Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1). J Biol Chem. 1992 Aug 15;267(23):16323-9. PMID: 1379595.

References